新扬州鸡IGF-1基因多态性与早期生长速度关系的研究   总被引：9，自引：2，他引：9  

王志跃  范刚  杨海明  汪张贵  盛东风  刘桂琼 《中国家禽》2004,26(24):9-12

以150只非同胞新扬州鸡为材料，采用PCR—RFLP法检测了该基因5’调控区DNA序列多态性，并运用线性模型统计方法分析了多态性与初生重和12周龄体重的关系。结果显示：新扬州鸡IGF-1基因5、调控区自然存在两种不同DNA序列，经。PstⅠ酶切后出现3种基因型(“-／-”、“-／ ”、“ ／ ”)，基因型分布符合哈代一温伯格定律。各基因型个体的初生重、12周龄体重的最小二乘均数存在“-／-”＞“ ／-”＞“ ／ ”的趋势，且“-／-”型个体的12周龄体重显著高于“ ／ ”型个体(P＜0．05)。  相似文献   

Mitochondrial DNA analysis of Nepalese domestic dwarf cattle Lulu*     

Kumiko TAKEDA  Masahiro SATOH  Shreeram P. NEOPANE  Bahadur S. KUWAR  Hari D. JOSHI  Nanda P. SHRESTHA  Hiroshi FUJISE  Mariko TASAI  Takahiro TAGAMI  Hirofumi HANADA 《Animal Science Journal》2004,75(2):103-110

Dwarf Lulu cattle, the only Bos Taurus type of cattle in Nepal, are raised under severe environments in the mountainous zone of that country. In the present study, the body measurement traits, cytogenetic and molecular genetic characteristics of the Lulu cattle are investigated. Blood samples were collected from 31 animals in four villages (altitudes 2590–3550 m) in the southern part of Mustang. The Lulu cattle had a normal karyotype with 2n = 60, XY or XX. Only one male examined had a large submetacentric X‐chromosome and a small submetacentric taurine type Y‐chromosome. The mitochodrial DNA (mtDNA) genotypes were analyzed by PCR mediated restriction fragment length polymorphisms, displacement (D)‐loop region PCR mediated single strand conformation polymorphisms, and D‐loop region sequences. Many base substitutions were found in the D‐loop region, suggesting that the Lulu cattle originated from at least 10 maternal lines. Three types of mtDNA from these cattle were found, the Bos taurus type (n = 23), the Bos indicus type (n = 6), and the Bos grunniens type (n = 2). In the village at the lowest altitude, four of the five cows were of the Bos indicus type. These results indicated that mtDNA types of the Lulu cattle mostly belong to Bos taurus, but have been hybridized with Bos indicus cattle in lower‐elevation regions in their maternal lineage.  相似文献   

微卫星DNA在动物亲子鉴定中的应用及研究进展     

吴艳  侯水生  肖红卫  刘小林 《现代畜牧兽医》2004,(10):38-39

本文概述了微卫星DNA进行亲子鉴定的基本原理与方法,将其用于亲子鉴定的优点以及研究进展,最后对未来的应用进行了展望。  相似文献   

A note on the genetic diversity of the vulnerable aquatic macrophyte Sparganium erectum and its congeners (Sparganiaceae) in rural wetland in Japan     

TEMPEI ISHII  YUICHIRO NAKAYAMA  MIKIO KOBAYASHI  HIROFUMI YAMAGUCHI 《Weed Biology and Management》2004,4(4):230-234

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Trichoderma Biocontrol of Colletotrichum acutatum and Botrytis cinerea and Survival in Strawberry   总被引：1，自引：2，他引：1  

Stanley Freeman  Dror Minz  Inna Kolesnik  Olga Barbul  Aida Zveibil  Marcel Maymon  Yehuda Nitzani  Benny Kirshner  Dalia Rav-David  Alon Bilu  Arnon Dag  Sharoni Shafir  Yigal Elad 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(4):361-370

Trichoderma isolates are known for their ability to control plant pathogens. It has been shown that various isolates of Trichoderma, including T. harzianum isolate T-39 from the commercial biological control product TRICHODEX, were effective in controlling anthracnose (Colletotrichum acutatum) and grey mould (Botrytis cinerea) in strawberry, under controlled and greenhouse conditions. Three selected Trichoderma strains, namely T-39, T-161 and T-166, were evaluated in large-scale experiments using different timing application and dosage rates for reduction of strawberry anthracnose and grey mould. All possible combinations of single, double or triple mixtures of Trichoderma strains, applied at 0.4% and 0.8% concentrations, and at 7 or 10 day intervals, resulted in reduction of anthracnose severity; the higher concentration (0.8%) was superior in control whether used with single isolates or as a result of combined application of two isolates, each at 0.4%. Only a few treatments resulted in significant control of grey mould. Isolates T-39 applied at 0.4% at 2 day intervals, T-166 at 0.4%, or T-161 combined with T-39 at 0.4% were as effective as the chemical fungicide fenhexamide. The survival dynamics of populations of the Trichoderma isolates (T-39, T-105, T-161 and T-166) applied separately was determined by dilution plating and isolates in the mixtures calculated according to the polymerase chain reaction (PCR) using repeat motif primers. The biocontrol isolates were identified to the respective species T. harzianum (T-39), T. hamatum (T-105), T. atroviride (T-161) and T. longibrachiatum (T-166), according to internal transcribed spacer sequence analysis.  相似文献   

DNA甲基化与基因组印记     

束婧婷 《畜牧兽医杂志》2005,24(6):18-20

综合论述了DNA甲基化与基因组印记的最新研究进展,论述了它们的生物学意义,并阐述了两者的关系.  相似文献   

H5亚型禽流感DNA疫苗质粒pCAGGoptiHA5对高致病力禽流感病毒攻击的免疫保护   总被引：5，自引：0，他引：5  

姜永萍  张洪波  李呈军  步志高  于康震  陈化兰 《畜牧兽医学报》2005,36(11):1178-1182

对H5亚型禽流感DNA疫苗质粒pCAGGoptiHA5的免疫效果进行了研究。pCAGGoptiHA5分别以100和10μg剂量一次或两次免疫3周龄SPF鸡，首次免疫后4周以同样剂量和途径进行第二次免疫，一次免疫后4周、两次免疫后2周分别用100LD50的HPAIV A／Goose／GuangDong／1／96（H5N1）鼻腔途径进行攻击，观察发病与死亡情况，分别于攻毒后第3、5、7天采集喉头及泄殖腔拭子进行病毒分离、滴定检测排毒情况，同时检测免疫后、攻毒前及攻毒后血清HI抗体、NT抗体以及AGP抗体的动态变化。结果，100μg pCAGGoptiHA5一次免疫、100μg pCAGGoptiHA5两次免疫以及10μg pCAGGoptiHA5两次免疫均可对免疫鸡形成100％完全保护（不发病、不致死、不排毒），10μg剂量pCAGGoptiHA5一次免疫可对免疫鸡形成100％的保护（不发病、不致死），结果表明，pCAGGoptiHA5作为疫苗效果良好、成本低廉，有望成为预防H5亚型高致病力禽流感的高效、安全新型基因工程疫苗。  相似文献   

Plasma NT‐proBNP and Cell‐Free DNA Concentrations after Prolonged Strenuous Exercise in Working Farm Dogs          下载免费PDF全文

H. Hunt  N. Cave  J. Bridges  K. Gedye  K. Hill 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2018,32(1):135-141

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Methyl donors dietary supplementation to gestating sows diet improves the growth rate of offspring and is associating with changes in expression and DNA methylation of insulin‐like growth factor‐1 gene          下载免费PDF全文

Yang Zhao  Yuedong Xuan  Daolin Mou  Hong Liu  Pan Zhou  Zhengfeng Fang  Lianqiang Che  Shengyu Xu  Bin Feng  Jian Li  Xuemei Jiang  Yan Lin  De Wu 《Journal of animal physiology and animal nutrition》2018,102(5):1340-1350

The study aimed to investigate the effects of maternal dietary methyl donors on the performance of sows and their offspring, and the associated hepatic insulin‐like growth factor‐1 (IGF‐1) expression of the offspring. A total of 24 multiparous sows were randomly fed the control (CON) or the CON diet supplemented with methyl donors (MD) at 3 g/kg betaine, 15 mg/kg folic acid, 400 mg/kg choline and 150 μg/kg VB₁₂, from mating until delivery. After farrowing, sows were fed a common lactation diet through a 28‐days lactation period and six litters per treatment were selected to be fed until at approximately 110 kg BW. Maternal MD supplementation resulted in greater birthweight (p < 0.05) and increased the piglet weights (p < 0.01) and litter weights (p < 0.05) at the age of day 28, compared with that in CON group. The offspring pigs in the MD group had greater ADG (p < 0.05) and tended to lower F:G ratio (p = 0.07) compared with that of CON group from day 28 to 180 of age. The offspring pigs from MD group had greater serum IGF‐1 concentrations and expressions of hepatic IGF‐1 gene and muscular IGF‐1 receptor (IGF‐1r) protein at birth (p < 0.05), and greater hepatic IGF‐1 protein (p = 0.03) and muscular IGF‐1r gene expressions (p < 0.05) at slaughter, than that from the CON group. Moreover, the methylation at the promoter of IGF‐1 gene in the liver of newborn piglets and finishing pigs was greater in the MD group than that of the CON group (p < 0.05). In conclusion, maternal MD supplementation throughout gestation could enhance the birthweight and postnatal growth rate of offspring, associated with an increased expression of the IGF‐1 gene and IGF‐1r, as well as the altered DNA methylation of IGF‐1 gene promotor.  相似文献   

BPA不影响卵母细胞减数分裂相关基因Dazl的甲基化   总被引：1，自引：0，他引：1  

章寒琼  张西锋  潘博  沈伟  李兰 《青岛农业大学学报(自然科学版)》2011,(2):83-87

为了探讨环境雌激素BPA对小鼠卵母细胞减数分裂相关基因Dazl甲基化的影响,本研究通过给孕鼠饮用含有BPA的水方式使胎鼠在发育过程中接触BPA,利用重亚硫酸盐测序法,分析了胎鼠生殖嵴卵母细胞不同发育时期Dazl甲基化水平的变化。结果显示:Dazl在减数分裂期间处于低甲基化水平,无论对照组或处理组均低于10%,说明Dazl的低甲基化对维持减数分裂的正常进行有重要作用;对照组与处理组的甲基化水平相当,差异不显著,说明本研究的BPA浓度不影响卵母细胞Dazl的甲基化水平。  相似文献